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VOL. 7, ISSUE 4 (2025)
Morpho-molecular characterisation of Allocanariomyces spp. causing die-back of chilli
Authors
Mounika Amplou, Savitha, A S, Ajithkumar, K, Yenjerappa, S T, Ramesh, G
Abstract
Die-back of chilli (Capsicum annuum L.) represents a complex
disease associated with multiple pathogenic organisms and emerged as a major
constraint to sustainable chilli production. The disease causes recurrent
outbreaks leading to substantial yield losses and posing a serious threat to crop
productivity. In the present investigation, a random roving survey was
conducted across the principal chilli-growing regions of Karnataka to determine
intensity of die-back. Symptomatic plant were collected from fields and
subjected to pathogen isolation on potato dextrose agar medium under aseptic
conditions. The cultural and morphological characterization of the fungal
isolate revealed colonies that were greyish green centre with white margin on
both the sides of the plate. Conidia were produced singly, typically
humicola-like and borne on the tips of hyphae. For molecular confirmation,
genomic DNA was extracted from pure cultures and the Internal Transcribed
Spacer (ITS) region of ribosomal DNA was amplified using universal primers
ITS-1 and ITS-4, resulted in an amplicon of approximately 560 base pairs. The
amplified product was sequenced and subjected to BLAST analysis against
sequences available in the NCBI GenBank database. The sequence exhibited high
similarity with reference sequences of Allocanariomyces sp., thereby
confirming its identity as the causal organism associated with chilli die-back
in the surveyed regions.
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Pages:106-109
How to cite this article:
Mounika Amplou, Savitha, A S, Ajithkumar, K, Yenjerappa, S T, Ramesh, G "Morpho-molecular characterisation of <i>Allocanariomyces</i> spp.<i> </i>causing die-back of chilli". International Journal of Ecology and Environmental Sciences, Vol 7, Issue 4, 2025, Pages 106-109
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