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VOL. 7, ISSUE 4 (2025)
Isolation, identification and antagonistic evaluation of Streptomyces spp. from rhizosphere soil against Aspergillus flavus
Authors
Sumeet Biradar, Gururaj Sunkad, Mallikarjun Kenganal, Pramesh D, Nagaraj M Naik, Hari Kishan Sudini
Abstract
Groundnut (Arachis hypogaea, L.) is one of the important legume
crops of tropical and semiarid tropical countries and known as “King of
Oilseeds” it is third largest oilseed produced in the world and second largest
in India. Preharvest and postharvest diseases are the
major constraints in the groundnut among which aflatoxin producing Aspergillus
flavus contamination is the major bottleneck. Biological control is one
of the eco-friendly and reliable approach for managing those constraints.
Therefore, in the present investigation rhizosphere soil was collected from the
seven districts of central and northern Karnataka and twenty-one isolates of Streptomyces
spp. isolates were obtained. Out of twenty-one, eleven isolates were confirmed
as Streptomyces spp. through Gram staining, aerial mycelium
characteristics and molecular characterization. Later these eleven isolates
were tested for their antagonistic potential against A. flavus by dual
culture assay under in vitro. The percentage inhibition of mycelial
growth ranged from 11.11 per cent to 61.11 per cent. Isolate LIN15 showed
highest (61.11 %) mycelium inhibition whereas, control showed zero per cent
inhibition against the pathogen.
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Pages:87-92
How to cite this article:
Sumeet Biradar, Gururaj Sunkad, Mallikarjun Kenganal, Pramesh D, Nagaraj M Naik, Hari Kishan Sudini "Isolation, identification and antagonistic evaluation of <i>Streptomyces</i> spp. from rhizosphere soil against <i>Aspergillus flavus</i>". International Journal of Ecology and Environmental Sciences, Vol 7, Issue 4, 2025, Pages 87-92
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