The present study aims to find out the effects of tartrazine in aquatic weeds in cultural conditions. For the study, the fresh plant samples were exposed to 1 and 2.5 mg/L of tartrazine in glass jars. Tartrazine is a well-known azo dye that also has weedicide properties. For the study, the different concentrations (1 mg/L-ST1) and (2.5 mg/L-ST2) of tartrazine were treated for 7 days. The biochemical composition such as total proteins, total lipids, total carbohydrates, and pigments like total chlorophyll were estimated at the end of exposure experiments. The dye was accumulated in the plant materials and imparted a yellowish colour to the leaves. The biochemical attributes showed significant (p<0.001) alterations with respect to the control. There was no germination in the test plants and which also showed a reduction in total biomass. The study confirmed the weedicide property of tartrazine and the indirect toxicity to other aquatic fauna such as herbivorous fishes.