Most of the soil born fungi are pathogens with the number of more than 8,000 species which cause diseases to agricultural crops. The agricultural plants are susceptible to these soil borne diseases that is the most concerning part. These fungi present a common threat to both agricultural production and the health of healthy and immuno-compromised individuals. Taken together, these relative fungi can cause huge economic losses to agriculture, loss of food for consumption, and serious, often fatal diseases in humans and animals. In the present study the soil samples were collected from different agricultural fields of Dehradun (Uttarakhand), India. The isolation of fungal isolates was done on SDA media by following serial dilution and spread plate technique at temperature and incubation period of 30 0C and 24 hours respectively. Based on the morphological and gram staining results a total of 9 different species of soil borne fungi were isolated, among which Rhizopus and Mucor were dominant. Further it was found that 3 different species of Aspergills viz. A. versicolor, A. glaucus and A. flavus were also identified. All three species of Aspergillus are considered as pathogens that damage the agronomically important crops and A. flavus is able to cause diseases in economically important crops, such as maize and peanuts, and to produce potent mycotoxins. It was concluded that the soils of the agricultural fields have a good diversity of fungal species and some of the isolates were pathogenic as per the available scientific literature. Hence the research provides the early alarm for the presence of pathogenic fungi that can be detrimental to economically important crops of the regions and thus the loss can be prevented by taking early preventive measures.