The present investigation aimed to degrade the sanitary napkins by microorganisms and its enzymatic activity. The invitro microbial degradation were performed under the controlled condition and soil degradation under the controlled environmental condition. The higher grade of degradation efficiency found on the organisms like Bacillus, Pseudomonas, Aspergillus and Fusarium. Lyophilized organisms were used to degrade the used napkinson dumpyards to prevent the environmental pollution impacts.