Background and objective: Bagrus bayad is a fish species that abundantly found in White Nile River. The present study was to assess the levels of heavy metals and their effects on enzyme activities in type of fish species. Materials and methods: Ethanol extract was taken for the determination of cholesterol, total lipid contents in fish muscles. While saline extract was used for the estimation of glucose, total protein, albumin, globulin and enzyme like GOT, GPT, LDH, CPK and amylase. The concentrations of heavy metals were carried out by the Flame atomic absorption spectrophotometer (UNICAM 929). Results: No significant difference was observed regarding GOT, LDH, ALP and amylase levels in both of the samples under the study. Whereas LDH level in site 1 (84.9) was found to be higher but not significantly different comparing to the control (80.4). The Cd and Cr levels in the liver and Pb, Cr and Fe in the muscle were found to significantly different to control. Conclusions: The accumulation of heavy metals in liver and muscles was greatly affected enzymes' activity of GOT, GPT, and LDH of the tested muscle extract.