Spiders are key components of all ecosystems in which they live and considered to be useful indicators of the overall species richness, health of terrestrial communities, Natural Pest Control, Good friend of Farmers. The present study is an attempt to investigate the spider faunal diversity in the selected habitats of Tropical butterfly conservatory (Butterfly Park), Tiruchirappalli, India. Guild structure analysis of the collected spiders revealed seven functional groups viz. stalkers, orb-web builders, ambushers, space-web builder, ground runners, foliage runners and sheet-web builders. The knowledge generated from the present study gives valuable and updated information on diversity of species of Tropical butterfly conservatory (Butterfly Park), Tiruchirappalli and the data can be used for future research on spider fauna.