Logo
International Journal of
Ecology and Environmental Sciences
ARCHIVES
VOL. 4, ISSUE 1 (2022)
Isolation of coomassie brilliant blue-G dye degrading bacteria from effluent, screening & evaluation of their dye decolorization activity
Authors
Pranali Jadhav, Kishori Tarfe
Abstract
Rapid industrialization has led to the accumulation of various toxic elements that harm the environment and ultimately affect human lives. Synthetic dyes are widely used because of cost-effective synthesis, high stability to light, temperature, etc. as compared to natural dyes. Textile dye effluents generally consist of toxic synthetic dyes which consist of 50%-70% of azo dyes. Bioremediation has become a key to dealing with hazardous pollutants. Various bacteria such as Bacillus subtilis, Aeromonas hydrophila, and Bacillus cereus, fungi & actinomycetes have been found to possess dye decolorizing activity. Bacteria from dye-contaminated effluent may possess dye decolorizing ability due to their adaptation to extreme environmental conditions. Media consisting of Coomassie brilliant blue-G at different concentrations ranging from 0.005%, 0.01%, and 1 % were used to isolate dye degrading organisms from effluent samples. Colony characteristics of organisms obtained from medium containing Coomassie brilliant blue-G were studied and biochemical tests i.e., IMViC, TSI were performed for identification. Dye decolorization assay was performed colorimetrically at 615 nm for concentrations of Coomassie brilliant blue-G ranging from 0.005%, 0.01%, and 1% every 24 hours. The percentage of dye decolorization activity was found to be 94%, 28%, and 0.07% for Coomassie brilliant blue-G for concentrations ranging from 0.005%, 0.01%, and 1% respectively. The probable organism isolated that helped in decolorization of 0.005% concentration may be of Gram-positive species, for 0.01% concentration may be Pantoea agglomerans and for 1% may be Escherichia sp. The probable isolates obtained need to be further investigated regarding various factors such as media composition affecting dye degradation & mechanism of dye degrading activity.
Download
Pages:116-124
How to cite this article:
Pranali Jadhav, Kishori Tarfe "Isolation of coomassie brilliant blue-G dye degrading bacteria from effluent, screening & evaluation of their dye decolorization activity". International Journal of Ecology and Environmental Sciences, Vol 4, Issue 1, 2022, Pages 116-124
Download Author Certificate

Please enter the email address corresponding to this article submission to download your certificate.