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VOL. 3, ISSUE 3 (2021)
Isolation, identification and characterization of microbes capable for bio remedial activities isolated from Jharia coalfield, Jharkhand
Authors
Amita Singh, Pawan Kumar, Navita Gupta Debasish Sahoo, Virendra Vaishnav
Abstract
The present study focused on identifying heavy metal degrading bacteria in soil sample collected from jharia coalfield sites Jharkhand. For isolation of bacteria from the soil sample was serially diluted tenfold; the highest dilution (10-6) sample was inoculated onto BBH agar plates and incubated at 37°C for 24 hrs. Colonies with diverse morphological features from the mixed cultures were sub cultured in order to obtain pure colonies. These colonies were then grown on nutrient agar containing diverse molarities of salts of heavy metals (Pb, Cu, Zn and Mn). The highest concentration of each heavy metal growth was observed were as follows: Zn-8mM, Cu-4mM, Pb-6mM and Mn-4mM. The bacteria having multiple tolerances were identified as Bacillus spp. on the basis of colony morphology, microscopic examination and biochemical tests. Bacillus licheniformis was identified through FAME GC. The tolerant isolate was further subjected to in vitro antibiotic cultural sensitivity assay and were found resistant to multiple antibiotics. The study is of significance in that such bacteria can be utilized for bioremediation of heavy metals in the environment particularly in the treatment of heavy metal contaminated soil.
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Pages:166-171
How to cite this article:
Amita Singh, Pawan Kumar, Navita Gupta Debasish Sahoo, Virendra Vaishnav "Isolation, identification and characterization of microbes capable for bio remedial activities isolated from Jharia coalfield, Jharkhand ". International Journal of Ecology and Environmental Sciences, Vol 3, Issue 3, 2021, Pages 166-171
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