Fruit rot disease caused by Fusarium musae is one of the post-harvest disease of banana and is adversely affects the fruit quality and the market value. In present study various antagonists were screened to know their bio efficacy in controlling the Fusarium fruit rot of banana.The highest per cent growth inhibition was found in the treatment of Trichoderma viride (77.52%) followed by Pseudomonas fluorescens in vitro. Whereas, under in vivo condition, T. viride both in pre inoculation (11.77%) and post-inoculation (11.43%) methods at 8th day after inoculation was found most potent antagonist in reducing the severity and it was remained at par with P. fluorescens.