The present investigations were carried out to study the effect of heavy metals viz. As and Pb on morphological and biochemical parameters and their uptake in Poplar hybrid (Populus ciliata x Populus maximowiczii) seedlings. Pot experiment was conducted on one-year-old cuttings. Experiment was laid out with 16 treatments consisting of various combinations of different levels of as and Pb at 0, 5, 10 and 20 ppm. Arsenic had significant influence on all the morphological parameters, whereas, lead significantly influenced seedling height, stem diameter, root dry weight and total dry biomass and had no significant impact on number of leaves, collar diameter and leaf area of Poplar hybrid. Biochemical parameters like chlorophyll content and total soluble sugar in leaves decreased with increasing level of as and Pb. The species was capable of tolerating the applied concentrations of as and Pb and could be a good candidate for planting in the heavy metal contaminated soils. The present investigations widen the scope for studying the effect of higher concentration of heavy metals beyond 20 ppm in future to confirm the phytoremediation ability of this Poplar hybrid species.