An investigation of abiotic parameters was under taken during October 2013-September 2015 in Khamti Guali beel of Dibru-Saikhowa Biosphere Reserve. Water temperature was recorded using a centigrade thermometer; pH and specific conductivity were recorded with the help of field probes; dissolved oxygen was estimated by the modified Winkler’s method; and the other abiotic parameters were analyzed by following A.P.H.A. (1992). Results revealed that, water temperature corroborated with the geographical location and sub-tropical nature of the sampled beel. Hydrogen ion values affirmed slightly acidic to alkaline nature of waters; specific conductivity recorded low ionic concentrations and thus, warranted inclusion of this sampled beel under ‘Class 1’ category of Talling and Talling. Dissolved oxygen indicated moderately oxygenated nature of waters and free carbon dioxide is attributed to its insufficient uptake by photo-autotrophs in the sampled floodplain lake. Total alkalinity and total hardness exhibited soft to hard water character; calcium content indicated ‘soft to moderately soft’ calcium poor waters. Magnesium, an important co-factor in enzymatic transformations resulted in the dominance of Calcium > Magnesium. Chloride concentrations corroborated with the natural waters and thus, indicated lack of influence of organic pollution caused by human impact. The sampled beel recorded low concentrations of dissolved organic matter, total dissolved solids and nutrients namely- phosphate, nitrate, sulphate and silicate during the study period.