All life forms require water which is essential for their survival. One of the major ecotoxicological issues that threaten most of the life forms in water bodies is pesticide contamination. The removal of pesticides in aquatic ecosystems has become an important area to reduce the risk to the organisms present. Fish is one of the important sources of protein and other elements to human beings. The fish Labeo rohita is taken to study the impact assessment of pesticide contamination in fish which is consumed by most of the people of Tamil Nadu. The samples were collected from fresh water bodies which are nearby to the agricultural field and analyzed for the nutritional value which is required by the human beings. The physical, chemical parameters and microbial analysis of the water samples were done. To assess the pesticide contamination the collected fish samples were then subjected to biochemical analyses. It is noticed that the contamination of inorganic pesticides in water results in the decreased level of prote-in and glycogen levels in fish which will lead to high mortality rate of fishes.