Hybrid tomato if propagated through vegetative method would be of great help to the farmers in reducing the cost of hybrid seeds. Auxin plays crucial role in vegetative propagation by inducing roots in stem cuttings. The objective of the present investigation was to study genetic variation in auxin induced root traits of twenty two tomato genotypes by estimating GCV, PCV, heritability and genetic advance. Stem cuttings of 25-30 days old tomato plants were treated with 500 ppm of IBA solution for 45 seconds to observe length of the longest root, number of lateral roots and fresh root weight. Analysis of variance indicated significant difference among the genotypes in respect of root length, number of lateral roots and fresh root weight. Experimental results revealed that the longest root was recorded in BT 10 (4.17 cm). Number of lateral roots varied from 12.23 to 47.53 with an average of 27.07. Fresh root weight ranged from 18.0 mg to 120.0 mg with an average of 60 mg. The phenotypic coefficient of variation (PCV) for all character was higher than genotypic coefficient of variation (GCV). The estimation of broad sense heritability was the highest for number of lateral roots (87.60). Genetic advance as % of mean was the highest for fresh root weight (87.29). Five genotypes having superior auxin induced rooting pattern were identified based on rooting index value.