Industrialization and globalization are the foremost cause of soil, air, and water pollution. Among all these, air pollution has produced much interest, later it has a main impact on the transboundary diffusion of pollutants worldwide. Mitigating air pollution is a great challenge, in such a situation, screening of plants as a bio-monitor is enormously significant. The present study has been considered to assess the Air Pollution Tolerance Index (APTI) and Anticipated Performance Index (API) of the common flora along highways. Five common plant species from amongst tree, herb and shrubs namely Eucalyptus spp. (myrtaceae), Mangifera indica (Anacardiaceae), Grewia optiva (malvaceae), Ficus roxburghii (moraceae) and Morus alba (moraceae) were carefully chosen growing frequently in the vicinity of the National Highway-88. APTI and API of selected plant species were examined by defining significant biochemical parameters like ascorbic acid, total chlorophyll content, leaf extract pH and relative water content. Besides, the selected vegetation was considered for physiological, economic, morphological as well as biological characteristics. It was found that two of the selected plants were sensitive to air pollution. Though, Mangifera indica and Ficus roxburghii showed the highest API and hence, suggested for plantation in a pollution stress area.