The field experiments were conducted with an application of human urine and cattle urine as nutrients sources. Ashgourd (Benincase hispida Thunb.) Cong.), french bean (Phaseolus vulgaris L.), pole bean (Phaseolus vulgaris L.) and pumpkin (Cucurbita maxima) which were grown as test crop. The experimental geographical area had been situated between 13o 292´ North latitude 77o 543´ East longitude and an altitude of 880 meters (2,890 ft) above mean sea level at Bangalore rural district (Karnataka, India). The experiment had 14 treatment combinations to study the effect of repeated application of human urine and cattle urine on yield and nutrient uptake of vegetable crops. Among the treatments application of recommended dose of nitrogen through human urine in three split doses plus gypsum recorded higher yield (39.2, 14.2, 17.4 and 38.7 t ha–1, for ashgourd, french bean, pole bean and pumpkin, respectively).