Since the very early civilization, rivers have been a vital source of livelihood for millions of people. Human use of river water had significant direct and indirect impacts upon fluvial environment in the form of river regulation. The processes of river regulation introduce short and long-term changes land-use and land-cover in morphology of the river basin. The present paper attempts to identify the spatial dimensions of river regulation in lower reaches of Silabati River and analyse the impact of human activities on morphology and ecological setting of the flood plains in parts of lower Silabati river basin, West Bengal, by using remote sensing and GIS techniques. The study is primary based upon field survey along with the consultation of toposheet and satellite imagery. The secondary information has been collected from Ghatal block development authority of the region. The key parameters considered for this change are interlinking channels, cross valley, meander scars, sinuosity index and water logged area. The study concludes that in-channel morphology is dominated by glides and relatively fast flowing features; whereas the effect of abstraction created in regulation section reaches is dominated by slow flowing pool type dynamics. The dynamics of the off-channel are also regulated by the human beings for reclaiming the land for agricultural purposes.