A field experiment was conducted during kharif season of 2017 to study the response of different fertility levels and split application of nitrogen on medium duration rice varieties at Agronomy Main Research Station, Odisha University of Agriculture and Technology, Bhubaneswar. Two fertility levels (100:50:50 kg N:P₂O₅:K₂O ha^-1 and 80:40:40 kg N:P₂O₅:K₂O ha^-1) each having two different splitting schedules of nitrogen (¼ basal + ½ tillering + ¼ PI) and (¼ basal + ½ tillering + 1/8 PI+ 1/8flowering) were allotted to the main plots whereas four rice varieties (Hasanta, Mrunalini, Ashutosh and Swarna) were allotted to the sub plots in a split plot design along with three replications. Growth variables such as Plant height, Maximum dry matter, Number of effective tillers and Total Nutrient Uptake were higher under application of 100:50:50 kg N:P₂O₅:K₂O ha^-1 with four splits of nitrogen. Among the varieties, Mrunalini recorded the maximum number of effective tillers, maximum accumulated dry matter, the highest total nutrient (NPK) uptake (237.26 kg ha^-1), net return (Rs. 42,765ha^-1 ) and benefit: cost ratio (2.66).