A survey of the spider fauna of Ramkrishna Mahavidyalaya campus and its adjacent area of Kailashahar, Unakoti District, Tripura, a part of Indo-Burma biodiversity hotspot region was carried out from July 2019 to June 2020 to assess the spider assemblages and their ecological guild structure. Methodology included active searching at all layers from ground level to tree canopy accessible for visual survey and photographic records were maintained. A total of 20 species belonging to 9 families viz. Araneidae, Thomisidae, Salticidae, Lycosidae, Sparassidae, Clubionidae, Oxyopidae, Tetragnathidae, Hersiliidae were recorded. Among these the most dominated family was Araneidae represented by 5 genera with 7 species. 5 families were represented by single species and 5 guild structures were noted. The present investigation revealed the potentiality of the study area as the reservoir of large spider diversity. The information collected may facilitate future initiatives of database preparation of these taxa for this region.