The study was conducted to evaluate the response of sugarcane variety CoC 24 to the application of bioinoculants viz., Gluconoacetobacter diazotrophicus, AM fungi and Azophos (Azospirillum and phosphobacteria), under different levels of N, P2O5 and K2O inorganic fertilizer in plant and ratoon crop. The results revealed that the application of mycor rhizae, G. diazotrophicus, Azospirillum and phosphobacteria significantly produced higher cane yield in plant crop. The application of Gluconoacetobacter diazotrophicus @10 kg + AM fungi + Azophos @10 kg + 75 % NPK recorded the maximum germination and tiller population and also maximum mean millable cane population of 1.32 lakhs /ha, cane yield (137.45 t/ha) and sugar yield (16.96 t/ha). Similar results were recorded with the ratoon crop. The applied bacterial sources helps in nitrogen fixation and also in continuous mobilizing and solubilization of nutrients and their persistence and colonization in soil is an added advantage and also enhances the soil fertility. The usage of these bioinoculants inturn reduces the inorganic fertilizer input and thereby reduces the cost of cultivation.