Weeds cause significant yield loss in crop plants. The use of herbicides is the economically viable technique for controlling weeds. However, the application of herbicides pollutes the soil, alter the microbial biota and exert a hazardous effect on human and animal health. The continuous use of specific herbicides for controlling weeds leads to the development of herbicide-resistant weeds. The use of natural metabolites from the plants exhibiting allelopathic potential is a novel strategy for sustainable weed management. Eucalyptus tereticornis is an excellent tree species with allelopathic properties. The study aimed to investigate different concentrations (5, 10 and 15%) of aqueous Eucalyptus tereticornis leaf extracts on the most noxious weed Echinochloa crus-galli. The dose-dependent inhibition was recorded in terms of germination percent, seedling growth and vigour. The aqueous leaf extract affected the emergence of the radicle. The inhibitory property of leaf extracts was due to the presence of allelopathic principles, which make Eucalyptus tereticornis as a promising candidate for obtaining bioherbicide.