Cassava (Manihot esculenta) is the well most important tuber crop in the tropics and sub tropics due to its drought tolerance, ability to grow in any soils and resistance to herbivore, cassava is well suited for cultivation by small and big farmers. However, it is short shelf life of the tuber due to Postharvest Physiological Deterioration (PPD) which limits cassava’s economic and industrial potential. Postharvest Physiological Deterioration in cassava tuber is rapid, begins within 5 to 24 hrs after harvest and its induce oxidative burst of Reactive Oxygen Species (ROS) which leads to produce hydrogen cyanide (HCN). The visible signs of deterioration are vascular streaking with a blue green discoloration that cause to be the tubers un edible and unmarketable. The present study was undertaken to evaluate 35 cassava genotypes for PPD tolerance. Tubers from different cassava genotypes were evaluated at 1,2,3,4 and 5 days after harvest for PPD. PPD was observed and cyanide and starch were analyzed each and every day. Two genotypes viz., CI-850 and YTP-1showed their supremacy in recording low levels of PPD 9.7 and 11.3 percent respectively even five days after harvest. These genotypes can be used as novel donor sources in breeding programmes aimed for developing PPD tolerant genotypes.