Experiment was conducted to study the effect of eCO₂ and eTemp on the carbohydrate, mid gut protein content and Carboxylesterae activity of S. litura after exposed to sublethal concentrations of insecticides (Spinosad, emamectin benzoate, thiodicarb, monocrotophos and fenvalerate). The results revealed that S. litura larvae treated with insecticides (emamectin, thiodicarb and monocrotophos) showed increased midgut protein and carbohydrate content with increase in CO₂ and temperatures and opposite trend was noted with spinosad and fenvalerate treated larva.