Resistance to biotic stress factors such as pests and diseases is the prime research topic across the world as it affects the yield of the crop significantly. Conventional plant breeding has been a successful technique to develop the biotic stress resistant cultivar but it is time taking and a laborious task. To speed up the breeding process and ensure site-specific breeding, scientists have been turning towards transgenic approaches to incorporate the desired gene into a plant variety. Many transgenic crops have been already commercialized and few are in the study phase. Bt cotton is one of the classical examples of a widely cultivated transgenic crop. Though transgenic crops are widely accepted by farmers across the globe, the argument of negative impact on health by the scientists is restricting the commercialization of crops. The advantages, disadvantages, and current status of transgenic breeding along with few successful studies are discussed in the current article.