Studies conducted on non-Apis bee fauna in eight districts of Karnataka revealed 44 species and 20 unidentified morphospecies in 23 genera. Three families of the Apoidea superfamily viz. Apidae, Megachilidae, Colletidae and Halictidae were represented in eight districts of Karnataka. The family Apidae was represented by three subfamilies viz., Xylocopinae (three genera and 23 species, and four unidentified species), Apinae (five species two unidentified species and two genera) and Nomadinae (One species). The subfamily Xylocopinae was represented with three tribes Xylocopini, Ceratini, and Allodapini, and Apinae was represented with two tribes Anthophorini and Melectini. The family Megachilidae was represented with single subfamily Megachilinae (14 species under five genera with two unidentified species) with two tribes Megachilini and Anthidiini. Family Halictidae was represented with 2 species and 11 unidentified species under 11 genera. Family Colletidae with single species. A checklist was prepared of the species documented in the present study.