In this research work, the Air pollution Tolerance Index of fifteen species collected from three different locations like Residential area, Industrial area and Heavy traffic area in Virudhunagar has been evaluated by analyzing biochemical parameters like Ascorbic acid, Chlorophyll, pH and Relative water content. The high values of Air pollution Tolerance Index were recorded in Moringa olifera, Azardiracta indica, Delonix regia, Mangifera indica, Eugenia jambolana and Fiscus religiosa for winter and summer season. The Air pollution Tolerance Index of plant species was also calculated by their Anticipated Performance Index together with other socio-economic and biochemical parameters. According to Anticipated performance index of most tolerant plant species for green belt development were Mangifera indica & Fiscus religiosa for road side and Azardiracta indica & Eugenia jambolona for industrial area.